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. 2019 Mar 26;9:5222. doi: 10.1038/s41598-019-41425-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DHE staining intensity in the retinal ganglion cell layer (RGCL, A), the inner nuclear layer (INL, B) and the outer nuclear layer (ONL, C) in aged wild-type and M1R−/− mice. DHE staining intensity was markedly elevated in the RGCL of aged M1R−/− mice (*P < 0.05) suggesting higher ROS levels. Pictures of DHE-stained retinal cryosections for aged wild-type (D; scale bar 50 μm) and M1R−/− mice (E; scale bar 50 μm).