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. 2019 Mar 26;9:5222. doi: 10.1038/s41598-019-41425-5

Table 1.

Primer sequences for qPCR of the genes examined in aged wild-type and M1R−/− mice.

TBP forward CTT CGT GCA AGA AAT GCT GAA T
TBP reverse CAG TTG TCC GTG GCT CTC TTA TT
NOX1 forward GGA GGA ATT AGG CAA AAT GGA TT
NOX1 reverse GCT GCA TGA CCA GCA ATG TT
NOX2 forward CCA ACT GGG ATA ACG AGT TCA
NOX2 reverse GAG AGT TTC AGC CAA GGC TTC
NOX4 forward TGT AAC AGA GGG AAA ACA GTT GGA
NOX4 reverse GTT CCG GTT ACT CAA ACT ATG AAG AGT
SOD1 forward CCA GTG CAG GAC CTC ATT TTA AT
SOD1 reverse TCT CCA ACA TGC CTC TCT TCA TC
SOD2 forward GCT CTG GCC AAG GGA GAT G
SOD2 reverse TGT CCC CCA CCA TTG AAC TT
SOD3 forward TTC TTG TTC TAC GGC TTG CTA CTG
SOD3 reverse AGC TGG ACT CCC CTG GAT TT
GPx1 forward CCT TGC CAA CAC CCA GTG A
GPx1 reverse CCG GAG ACC AAA TGA TGT ACT TG
HO-1 forward GGT GAT GCT GAC AGA GGA ACA C
HO-1 reverse TAG CAG GCC TCT GAC GAA GTG
Catalase forward CAA GTA CAA CGC TGA GAA GCC TAA G
Catalase reverse CCC TTC GCA GCC ATG TG
SIRT1 forward GCC AAA CTT TGT TGT AAC CCT GTA
SIRT1 reverse TGG TGG CAA CTC TGA TAA ATG AA
SIRT6 forward TGG CCC CCA AGT TTG ACA
SIRT6 reverse GCT GAA CCA GGG CCA TGT
FOXO1 forward GCG GGC TGG AAG AAT TCA A
FOXO1 reverse TTC CTT CAT TCT GCA CTC GAA TAA