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. 2019 Mar 26;9:5119. doi: 10.1038/s41598-019-41492-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activity of selected A domains within the “wild type” or mutated tri-modular construct of TycC. (a) Schematic depiction of the construct with amino acids attached to the PPE arms. Colors as in Fig. 2a. (b) Coomassie-stained SDS-gel showing the indicated purified proteins. (c–f) Graphs showing k_cat values for the indicated constructs in the presence of the indicated amino acids, calculated using the online PP_i release assay after subtraction of the background (=no substrate) value. Amino acids were added at 1 mM, enzymes at 0.5 µM. Data represent the mean (±standard deviation, SD) of three independent experiments.