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. 2019 Mar 26;9:5184. doi: 10.1038/s41598-019-40163-y

Figure 2.

Figure 2

Incorporation of BM-MSC-Mt into STZ-PTECs, and the anti-degenerative effects of BM-MSCs and BM-MSC-Mt in vitro. (a) Time-lapse images of Mt transfer from MtDsRed2-MSCs to STZ-PTECs. Images were obtained 4, 5 and 6 h after commencing time-lapse observations. Panels on the right show magnified images of the left panels. White arrows track the same DsRed2-Mt. M: MtDsRed2-MSCs, P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. (b) Time-lapse images of the incorporation of isolated Mt (BM-MSC-Mt) into STZ-PTECs. Images were obtained at 10 and 15 min and 4 h after commencing time-lapse observations. Panels on the right show magnified images of left panels. White arrows and arrowheads track the same DsRed2-Mt. P: PTECs derived from STZ rats (STZ-PTECs). Scale bar, 25 µm. (c) Phase contrast observations of Control-PTECs and STZ-PTECs cultured with or without BM-MSCs and BM-MSC-Mt. Images were obtained 12, 48 and 96 h after commencing the co-culture with BM-MSCs or BM-MSC-Mt. Scale bar, 100 µm.