Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 26;9:5184. doi: 10.1038/s41598-019-40163-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Anti-apoptotic effects of BM-MSCs and BM-MSC-Mt in vitro. (a) Immunofluorescence images of LTL (green) in Control-PTECs and STZ-PTECs cultured with or without BM-MSCs and BM-MSC-Mt. Images were obtained 96 h after commencing the co-culture with MtDsRed2-MSCs or MtDsRed2-MSC-Mt (red). Nuclei are counterstained with DAPI (blue). White arrows indicate deformed and shrunken nuclei. The white arrows in the insets indicate incorporated BM-MSC-Mt in STZ-PTECs. Experiments were repeated three times independently. Scale bar, 40 μm; 10 µm in insets. (b) Quantification of LTL aggregation. Data are shown as mean ± s.e.m. (Control-PTECs, n = 6; STZ-PTECs, n = 8; STZ-PTECs-MtDsRed2-MSCs, n = 4; STZ-PTECs-MtDsRed2-MSC-Mt, n = 4). *P < 0.05 Control-PTECs vs. STZ-PTECs, and Control-PTECs vs. STZ-PTECs-MtDsRed2-MSC-Mt; ^†P < 0.05 STZ-PTECs vs. STZ-PTECs-MtDsRed2-MSCs, and STZ-PTECs vs. STZ-PTECs-MtDsRed2-MSC-Mt; ^‡P < 0.05 STZ-PTECs-MtDsRed2-MSCs vs. STZ-PTECs-MtDsRed2-MSC-Mt using ANOVA corrected with the Bonferroni coefficient. (c) TUNEL staining (green) of Control-PTECs and STZ-PTECs cultured without or with BM-MSCs and BM-MSC-Mt. Images were obtained 96 h after commencing the co-culture with MtDsRed2-MSCs or MtDsRed2-MSC-Mt (red). Nuclei are counterstained with DAPI (blue). White arrows in insets (i) and (ii) show incorporated DsRed2-Mt in STZ-PTECs. White dotted lines in the insets show the outline of individual STZ-PTECs. Scale bar, 40 μm; 10 µm in insets. Experiments were repeated three times independently. (d) Quantification of TUNEL-positive apoptotic cells in Control-PTECs and STZ-PTECs cultured without or with BM-MSCs and BM-MSC-Mt. Data are shown as mean ± s.e.m. (Control-PTECs, n = 6; STZ-PTECs, n = 10; STZ-PTECs-MtDsRed2-MSCs, n = 4; STZ-PTECs-MtDsRed2-MSC-Mt, n = 8). *P < 0.05 Control-PTECs vs. STZ-PTECs; ^†P < 0.05 STZ-PTECs vs. STZ-PTECs-MtDsRed2-MSCs, and STZ-PTECs vs. STZ-PTECs-MtDsRed2-MSC-Mt using ANOVA corrected with the Bonferroni coefficient.