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. 2019 Mar 26;9:5184. doi: 10.1038/s41598-019-40163-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of Mt-related factors and ROS production in STZ-PTECs by the addition of BM-MSC-Mt in vitro. (a) Expression of SOD2 and Bcl-2 in the Mt-enriched fraction of STZ-PTECs by the addition of BM-MSC-Mt for 24 h (n = 4 per group). Cropped images of immunoblots are shown; full-length blots are shown in Supplementary Fig. S7a. (b) Densitometric analysis of the expression of SOD2 in (a). Data are shown as mean ± s.e.m. (n = 4 per group). *P < 0.05 STZ-PTECs-Mt (–) vs. STZ-PTECs-Mt (+) using the unpaired t-test. (c) Densitometric analysis of the expression of Bcl-2 in (a). Data are shown as mean ± s.e.m. (n = 4 per group). *P < 0.05 STZ-PTECs-Mt (–) vs. STZ-PTECs-Mt (+) using the unpaired t-test. (d) Fluorescence images of ROS production in STZ-PTECs co-cultured with or without BM-MSC-Mt. Nuclei are counterstained with DAPI (blue). Scale bar, 50 μm. (e) Quantification of ROS production in STZ-PTECs by the addition of BM-MSC-Mt. Data are shown as mean ± s.e.m. (n = 5 per group). *P < 0.05 STZ-PTECs-Mt (−) vs. STZ-PTECs-Mt (+) using the unpaired t-test.