Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 26;9:5184. doi: 10.1038/s41598-019-40163-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Expression of Mt-related factors, ROS production, and expression of protein and glucose transporters in NRK-52E cells following the addition of BM-MSC-Mt or Mt isolated from NIH-3T3 cells (3T3-Mt). NRK-52E cells were cultured in low glucose (LG) or high glucose (HG) conditions. (a) Expression of Bcl-2 and Bax in NRK-52E cells. Cropped images of immunoblots are shown; full-length blots are shown in Supplementary Fig. S7b. (b) Immunofluorescence images of PGC-1α (green) in NRK-52E cells. Nuclei are counterstained with DAPI (blue). Scale bar, 25 μm. (c) Fluorescence images of ROS production in NRK-52E cells. Nuclei are counterstained with DAPI (blue). Scale bar, 25 μm. Quantification of ROS production is shown in the right panel as the mean ± s.e.m. (n = 10 images per group). *P < 0.05 LG vs. HG-none; ^†P < 0.05 HG-none vs. HG-MSC-Mt using ANOVA corrected with the Bonferroni coefficient. (d) Immunofluorescence images of megalin (green) in NRK-52E cells. Nuclei are counterstained with DAPI (blue). Scale bar, 25 μm. Quantification of megalin expression is shown in the right panel as the mean ± s.e.m. (n = 10 images per group). *P < 0.05 LG vs. HG-none, LG vs. HG-MSC-Mt, and LG vs. HG-3T3-Mt; ^†P < 0.05 HG-none vs. HG-MSC-Mt, and HG-none vs. HG-3T3-Mt; ^‡P < 0.05 HG-MSC-Mt vs. HG-3T3-Mt using ANOVA corrected with the Bonferroni coefficient. (e) Immunofluorescence images of SGLT2 (green) in NRK-52E cells. Nuclei are counterstained with DAPI (blue). Scale bar, 25 μm. Quantification of SGLT2 expression is shown in the right panel as the mean ± s.e.m. (n = 10 images per group). *P < 0.05 LG vs. HG-none, and LG vs. HG-MSC-Mt; ^†P < 0.05 HG-none vs. HG-MSC-Mt using ANOVA corrected with the Bonferroni coefficient.