Figure 4.
m6dA and gene transcription. (a) CHM1 cell line gene expression from gene regions with (+) and without (−) m6dA identified with SMRT-seq. Transcripts are significantly higher in exons, introns and promoters with m6dA (*P-value < 2.2 × 10−16, Wilcox test). (TPM) transcripts per million. (b) AK1 cell line gene expression from gene regions with (+) and without (−) m6dA identified with SMRT-seq. Transcripts are significantly higher in exons, introns and promoters with m6dA (*P-value < 2.2 × 10−16, Wilcox test). (TPM) transcripts per million. (c) Overlap between genes with differentially methylated haplotypes and genes with allele specific gene expression in AK1 cells (P-value 9.1 × 10−15, hypergeometric test). (d) Pathways enriched for genes with differentially methylated haplotypes, genes with allele specific gene expression and their overlap in AK1 cells. (FDR) False discovery rate.