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. 2019 Mar 26;9:5188. doi: 10.1038/s41598-019-41735-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCA score images. The PC 1 scores (a) separate the data into pixels with high or low CH/CH₂ stretching intensity, while the PC 2 scores (b) separate the data into pixels with high aromatic contribution or high CH/CH₂ stretching intensity. The PC 3 scores (c) separate the data into pixels with high contributions from lipophilic extractives or cell wall polymers. Samples from left to right are week 0, 2, 4, 6 and 8. The size of each individual sample image is 70 × 70 μm.