Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 26;9:5194. doi: 10.1038/s41598-019-41001-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Promoter replacement strategy to assess effects of dacB operon repression using two independent anhydrotetracycline (aTc)-responsive repressors. (A) The native promoter of the dacB operon was replaced with pmycTET (orange box). Repressors (pMC1s, panels on left or pTEK4S-0x, panels on right) were then introduced, independently, into this strain to yield strains dacB TetON_s and dacB TetOFF, respectively. In the absence of aTc, gene expression should be off or on, respectively. (B,C) Expression of dacB in the absence (0) and presence (100 ng/ml) aTc. Transcript levels, for dacB, were measured during late logarithmic growth (18 hours) and normalized against sigA levels. (D,E) Repression of the dacB operon (red lines) resulted in a decreased growth rate. Strains were grown in 7H9 supplemented with glucose salts and Tween 80. Samples were removed at 3 hourly intervals to determine optical densities at 600 nm.