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. 2019 Mar 26;9:5147. doi: 10.1038/s41598-019-41643-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative size distribution of RBC aggregates observed by conventional optical microscopy under normal conditions and influencing by polymeric NP 600 nm (a), TiO₂ RODI 250 nm NP (b), TiO₂ Hombitan AN 180 nm NP (c), TiO₂ 15 nm NP (d), ZnO 270 nm NP (e) and ND 100 nm (f). The RBC aggregates are encircled and indicated with arrows. (g) Distribution of RBC aggregates by occupied area based on the quantitative assessment of images in (a–f).