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. 2019 Mar 26;9:5210. doi: 10.1038/s41598-019-41569-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Unfolding force distributions for utrophin terminal constructs reveal markedly different mechanical behaviors. (a–c) Plots of the probability distribution of unfolding force vs the unfolding force for Utr NT-R10 (a), Utr-R11-CT (b) and full-length utrophin (c) collected from 3 batches of 300–500 successful pulls per batch. Here, ‘N’ represents the unfolding event count. For example, the distribution corresponding to N = 4 represents the distribution of the 4th unfolding event.