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. 2019 Mar 27;39(13):2441–2458. doi: 10.1523/JNEUROSCI.2121-18.2019

Figure 6.

Figure 6.

Hippocampal excitatory synapse characterization of WT, pGFAP-BDNF, 5xFAD, and 5xF:pGB mice. A, Confocal image of PSD-95 immunofluorescence in CA1 stratum radiatum of 8-month-old WT, pGFAP-BDNF, 5xFAD, and 5xF:pGB mice. Scale bar, 10 μm. B, Quantification of the number of PSD-95-positive puncta per field. One-way ANOVA: F(3,16) = 14.90, p < 0.001. C, Synaptophysin immunofluorescence. Scale bar, 10 μm. D, Quantification of the number of synaptophysin-positive puncta per field. One-way ANOVA: F(3,16) = 7.194, p < 0.0028. E, Confocal image of a double PSD-95 (green) and phosphoTrkBY816 (red) immunofluorescence in CA1 stratum radiatum of 8-month-old WT, pGFAP-BDNF, 5xFAD, and 5xF:pGB mice. Scale bar, 10 μm. F, Quantification of the number of PSD-95/phosphoTrkBY816-positive puncta per field. One-way ANOVA: F(3,26) = 5.303, p < 0.01. G, Electronic microscopy imaging of excitatory synapses in the stratum radiatum of the CA1 in 8-month-old WT, pGFAP-BDNF, 5xFAD, and 5xF:pGB mice. Asterisks indicate the presynaptic component. Black arrows indicate the postsynaptic component. Scale bar, 0.3 μm. H, Quantification of the PSD area. One-way ANOVA: F(3,253) = 17.16, p < 0.001. I, Quantification of the number of presynaptic vesicles per synapse. One-way ANOVA: F(3,201) = 9.18, p < 0.001. One-way ANOVA with Tukey's test as a post hoc was used. Data are mean ± SEM. B, D, F, H, I, *p < 0.05; ***p < 0.001; compared with WT mice. $$p < 0.01; $$$p < 0.001; compared with 5xFAD mice. B, D, F, Two pictures per slice and 3 slices per mouse were taken. B, D, n = 5/genotype. F, n = 7–9/genotype. H, I, 51 ± 3 excitatory synapses from 3 different mice/genotype were evaluated.