Figure 4. Gene expression associated with cell cycle of bleomycin (BLM) treatment in MLE-12 cells. The MLE-12 cells were treated with BLM (1–10 µg/mL) for 24 hours. Measured the p21 (A), p53 (B), and proliferating cell nuclear antigen (Pcna ) (C) at the mRNA levels, which were associated with cell cycle. BLM exposed MLE-12 cells were measured expression of Slfn1 (D), Slfn2 (E), and Slfn4 (F) by quantitative real-time reverse transcription-polymerase chain reaction analysis.