Fig 1.

Experimental schemas. (a) Female littermates were exposed to isoflurane (Iso) or sham conditions at postnatal Day 4 (P4) and P7 (control, n=12; P4 Iso, n=12; P7 Iso, n=14). All groups underwent behavioural testing starting at P35. (b) Females were exposed to Iso at P4 (n=5) or P7 (n=5) or sham (n=4 for both P4 and P7 control groups) and perfused 18–24 h later. Brain tissue was stained with Fluoro-Jade C (FJC) to detect neurodegeneration. (c) Fresh brain tissue was dissected from females and males at P4 and P7 (n=5 per group) for protein quantification.