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. 2019 Jan 31;122(4):490–499. doi: 10.1016/j.bja.2018.12.008

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Fig 4 — Isoflurane induces higher rates of acute neurodegeneration in P4 females than P7 females in the hippocampus and laterodorsal thalamus. (a) Representative image of Fluoro-Jade C (FJC) staining in the laterodorsal thalamus of a P4 isoflurane-exposed rat, 10× (inset, 40×). (b) Number of neurodegenerating cells, fluorescently labelled with FJC+, per um³ in the hippocampus. Two-way ANOVA shows effect of treatment (P=0.0025) but not age (P=0.1516); no interaction (P=0.0588). Post hoc Sidak’s multiple comparison test shows significantly higher cell death in P4 Iso compared with P7 Iso (P=0.0288). (c) FJC+ cells in the laterodorsal thalamus. Two-way ANOVA shows effect of age (P=0.0018) and treatment (P<0.0001), interaction P=0.002. (d) FJC+ cells in the mediodorsal thalamus. Two-way ANOVA shows effect of treatment (P=0.0007) but not age (P=0.2055); no interaction (P=0.2052). Mean and standard deviation shown. * P<0.05, † P<0.001, ns = not significant.