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. 2019 Mar 20;10:277. doi: 10.3389/fpls.2019.00277

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Copyright © 2019 Deng, Achor, Exteberria, Yu, Du, Stanton, Liang and Gmitter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photomicrographs of transverse section showing comparative anatomical changes of HLB-affected citrus lamina. (A–H) Epifluorescence micrograph of anatomical characters through 20× objective lens. (A) HLB-free SB mandarin; (B) “Bearss” lemon; (C) SB mandarin; (D) “Valencia” sweet orange; (E) LB8-1 mandarin; (F) LB8-2 mandarin; (G) LB8-15 mandarin; (H) LB9-13 mandarin; and (I) light micrograph through 40× objective lens with methylene blue-azure A solution and basic fuchsin solution staining, LB8-15 mandarin. The red arrow in image (I) indicates the starch granules in a light micrograph. Scale bars = 20 μm. Abbreviations: UC, upper cuticle; UE, upper epidermis; PPC, palisade parenchyma cell; SPC, spongy parenchyma cell; ES, intercellular air empty space; LE, lower epidermis; and LC, lower cuticle.