Monocytes show a shorter surface MHC I half-life than DCs. (A) MHC I surface turnover of monocytes, imDCs, and mDCs examined at the indicated time points over 4 hours (red histograms). (B) Newly expressed MHC I on monocytes, imDCs, and mDCs examined at the indicated time points over 4 hours (light red histograms). The 4°C control was incubated for 4 hours and is shown in blue histograms. Graphs in panels A and B indicate the mean fluorescence intensity (MFI) of MHC I expression normalized to MFI values of time point 0 (means ± 95% CI, n = 6 for monocytes, imDC, and mDCs; 1 out of 4 independent experiments). **P ≤ .005; *P ≤ .05; Kruskal-Wallis test with Dunn’s correction for multiple comparison.