Figure 5.

DHA suppresses LPS-induced TNF-α mRNA expression in macrophages but does not affect LPS-induced RANKL mRNA expression in osteoblasts. (A) Osteoblasts from cranial bone were incubated with PBS, LPS, LPS+DHA with or without AH7614, or DHA alone. After 24-h culture, total RNA was isolated and RANKL mRNA levels were determined using real-time RT-PCR. (B) Peritoneal macrophages were cultured with PBS, LPS, LPS+DHA with or without AH7614 or DHA alone. After 24-h culture, total RNA was isolated and TNF-α mRNA levels were determined using real-time RT-PCR. RANKL and TNF-α mRNA levels were normalized to that of GAPDH. Data are expressed as means ± SD (n = 4; ^**p < 0.01, ^*p < 0.05). Differences were determined by one-way ANOVA and Turkey-Kramer tests.