KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-phospho-γH2A.X(S139) | Cell Signaling | Cat#2577; Lot 11; RRID: AB_2118010 |
| Mouse BrdU antibody | BD Biosciences | Cat#347580; Clone B44; Lot: 6042756; RRID: AB_400326 |
| Chemicals, Peptides, and Reoombinant Proteins | ||
| CDK1 inhibitor RO3306 | Sigma-Aldrich | Cat#SML0569; CAS 872573-93-8 |
| CDK2 inhibitor III | EMD Millipore | Cat#238803; CAS 199986-75-9 |
| Aphidicolin | Cayman Chemical | Cat#14007; CAS 38966-21-1 |
| Hydroxyurea | Sigma Aldrich | Cat#H8627; CAS 127-07-1 |
| Neocarzinostatin | Sigma-Aldrich | Cat#N9162; CAS 9014-02-2 |
| Ku-60019 (ATMi) | Cayman Chemical | Cat#17502; CAS 925701-46-8 |
| NU-7441 (DNA-PKi) | Cayman Chemical | Cat#14881; CAS 503468-05-9 |
| Chir-124 (Chk1i) | Cayman Chemical | Cat#16553; CAS 405168-58-3 |
| MK-1775 (Wee1i) | Cayman Chemical | Cat#21266; CAS 955365-80-7 |
| AZ-20 (ATRi) | Cayman Chemical | Cat#17589; CAS 1233339-22-4 |
| Critical Commercial Assays | ||
| Click-iT EdU Alexa Fluor 647 Imaging Kit | ThermoFisher Scientific | Cat#10340 |
| Experimental Models: Cell Lines | ||
| Human: MCF-10A | ATCC | ATCC #CRL-10317, RRID:CVCL_0598 |
| Human: BJ-5ta | ATCC | ATCC #CRL-4001 |
| Recombinant DNA | ||
| CSII-pEF-H2B-mTurquoise | Spencer et al., 2013 | N/A |
| CSII-pEF-DHB(aa994–1087)-mVenus | Hahn et al., 2009 | N/A |
| CSII-pEF-Geminin(aa1–110)-mCherry | Sakaue-Sawano et al., 2008. | N/A |
| Software and Algorithms | ||
| Cell tracking and cell analysis | Cappell et al., 2016 | N/A |