Figure 4. The CBM/NF-κB pathway is critical for AGTR1-dependent cell proliferation.

A and B, proliferation of ZR75-AGTR1 and BT549 cells in 2D, ± IKK-VI, as assessed using the quantitative IncuCyte system (mean ± SEM, n=4; **, p<0.01; ***, p<0.001). C and D, proliferation of the ZR75-AGTR1 and BT549 cell lines ± CARMA3 or MALT1 knockdown (mean SEM, n=3; ***, p<0.001; ****, p<0.0001). E and F, growth of ZR75-AGTR1 and BT549 spheroids ± IKK-VI (mean ± SEM, n=12). G and H, growth of ZR75-AGTR1 and BT549 spheroids ± Bcl10 knockdown (mean ± SEM, n=12).