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. 2019 Mar;189(3):590–603. doi: 10.1016/j.ajpath.2018.11.010

Figure 2.

Figure 2

Changes in Wnt gene expression in macrophages with Wls deletion. Real-time quantitative RT-PCR for a panel of Wnt ligands on bone marrow–derived macrophages (BMDMs) from WT and Mac-KO mice shows expression of all 16 Wnt genes. However, several Wnt genes (Wnt1, Wnt2, Wnt2b, Wnt3, Wnt4, Wnt5b, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt9a, Wnt9b, Wnt10a, Wnt11, and Wnt16) are up-regulated manyfold in the baseline Mac-KO BMDMs compared with BMDMs from WT mice. Wnt5a and Wnt10b are notably down-regulated, whereas there is no change in the expression of Wnt8b. The expression of each Wnt gene is normalized to glyceraldehyde-3-phosphate dehydrogenase and expressed as fold-change in Mac-KO over WT mice. Three Mac-KO or WT mice were used for isolating and differentiating BMDMs.