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Immunofluorescence analysis verifies increased ductular reaction in the Mac-KO mice after 14 days of DDC diet. Immunofluorescence staining was performed for transcription factor SOX-9 (Sox9) and cytokeratin 19 (CK19) to label ductular reaction, which is enhanced in the Mac-KO mice compared with WT mice after 14 days of DDC diet. DAPI was used as nuclear counterstain. Original magnification, ×200.
