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. 2019 Mar;189(3):590–603. doi: 10.1016/j.ajpath.2018.11.010

Figure 6.

Figure 6

Increased cell death, decreased proliferation, but comparable fibrosis in Mac-KO versus WT mice after 14 days of DDC diet. A: Staining for Sirius Red (top panels) shows comparable positivity in the Mac-KO and WT mice after 14 days of DDC diet. Immunohistochemistry for α-smooth muscle actin (α-SMA; bottom panels) to identify activated myofibroblasts also shows comparable numbers of positive cells in the Mac-KO and WT mice after 14 days of DDC diet. B: Quantification of both Sirius Red staining and number of α-SMA–positive cells in the Mac-KO and WT mice after 14 days of DDC diet shows insignificant differences between the two groups. C: Increased terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)–positive hepatocytes (arrowheads) in Mac-KO compared with WT mice after 14 days of DDC diet. D: Quantification of the number of TUNEL-positive cells per ×20 field shows a significant increase in the number of positive cells in Mac-KO mice after 14 days of DDC compared with WT mice. E: Decreased Ki-67–positive hepatocytes in Mac-KO compared with WT mice denotes fewer proliferating cells in Mac-KO mice after 14 days of DDC. F: Quantification of the number of Ki-67–positive cells per ×20 field shows a significant increase in the number of positive cells in Mac-KO mice after 14 days of DDC compared with WT mice. Data are expressed as means ± SD. n = 3 mice. ∗P < 0.05. Original magnifications: ×100 (A and E); ×200 (C). IOD, integrated optical density.