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. 2019 Mar;189(3):590–603. doi: 10.1016/j.ajpath.2018.11.010

Table 1.

Primers and PCR Assays Used

Gene Forward primer Reverse primer Vendor/assay ID
Arg1 Primer mix: FAM Bio-Rad Laboratories, Inc./qMmuCIP0034715
Arg1 5′-CAGAAGAATGGAAGAGTCAG-3′ 5′-CAGATATGCAGGGAGTCACC-3′ Integrated DNA Technologies, Inc., Coralville, IA
B2M Primer mix Bio-Rad Laboratories, Inc./qMmuCID0040553
B2M Primer mix: Hex Bio-Rad Laboratories, Inc./qMmuCIP0042770
Ccl2 5′-TAGGCTGGAGGGCTACAAGA-3′ 5′-TAGGTTCTGATCTCATTTGGTTCTG-3′ Integrated DNA Technologies, Inc.
Ccl3 Primer mix Bio-Rad Laboratories, Inc./qMmuCED0044190
Ccl4 Primer mix Bio-Rad Laboratories, Inc./qMmuCED0044850
Ccl5 5′-CGAAGGAACCGCCAAGTG-3′ 5′-CTAGAGCAAGCGATGACAGG-3′ Integrated DNA Technologies, Inc.
Cxcl1 Primer mix Bio-Rad Laboratories, Inc./qMmuCED0047655
Cxcl16 5′-AGCAAGATGACAGACAGCAAGAAG-3′ 5′-GCAGTGAGGAAGAAGACAATGGC-3′ Integrated DNA Technologies, Inc.
Sele 5′-TGCAGTTCTGACGTGTGGTG-3′ 5′-AACAAGGTAGAGCAATGAGGACGAT-3′ Integrated DNA Technologies, Inc.
Il6 5′-TGGAGTCACAGAAGGAGTGGCTAAG-3′ 5′-TCTGACCACAGTGAGGAATGTCCAC-3′ Integrated DNA Technologies, Inc.
Mgl1 Primer mix Bio-Rad Laboratories, Inc./qMmuCIP0036620
Mrc1 Primer mix Bio-Rad Laboratories, Inc./qMmuCID0012670
Nos2 Primer mix: Cy5 Bio-Rad Laboratories, Inc./qMmuCIP0035502
Nos2 5′-CTGGAGGAGCTCCTGCCTCATG-3′ 5′-GCAGCATCCCCTCTGATGGTG-3′ Integrated DNA Technologies, Inc.
Tnfa Prime mix Mm.PT.58.12575861 Integrated DNA Technologies, Inc./Mm.PT.58.12575861
Vcam1 5′-GCTCTGTGGGTTTTGAGGATGA-3′ 5′-GGATCTTCAGGGAATGAGTAGACC-3′ Integrated DNA Technologies, Inc.
Chil3 5′-TCACAGGTCTGGCAATTCTTCTG-3′ 5′-TTTGTCCTTAGGAGGGCTTCCTC-3′ Integrated DNA Technologies, Inc.