Supplemental Figure S2.

Limit of detection for tracking of a known clone at low level. Dilutions of 1%, 0.1%, 0.01%, 0.001%, and 0.0001%. Four replicates are shown for each to assess reproducibility of detection. The known clonal sequence could be consistently detected in all replicates down to the 0.001% dilution. Higher variability with respect to the percentage of the index clone is seen at levels beyond 0.01%. At the 0.0001% dilution, the clonal sequence is detected in only three of four replicates.