Supplemental Figure S3.

Comparison of total read counts by primer set and sample type. A: Comparison of total read counts in the characterization subset stratified by primer set. No significant difference is seen among primer sets. Higher number of outliers in the FR1 subset is related to the higher number of samples sequenced with FR1. B: Comparison of final read counts of formalin-fixed, paraffin-embedded (FFPE) samples compared with non-FFPE samples (blood, bone marrow aspirates). The FFPE samples often generate lower read counts but remain >100,000 in most cases. n = 775 (A); n = 51 (B, FFPE samples); n = 724 (B, non-FFPE samples).