Fig. 3.

Expression of AtGFAT1 affects the HBP flux in Arabidopsis. (A) Quantitative RT-PCR analysis for AtGFAT1 transcript levels. Total RNA was extracted from 12-day-old seedlings of the indicated genotype grown under normal conditions or treated for 6 h with 50 ng ml⁻¹ Tm. The AtGFAT1 expression levels were normalized to transcript levels for the reference gene ACTIN2, and then to AtGFAT1 transcript level in wild-type plants grown under normal conditions, which was set to 1. Values represent mean ±SE from three independent repeats. GlcN (B) and UDP-GlcNAc (C) contents in 12-day-old seedlings of the indicated genotype grown under normal conditions or treated for 6 h with 50 ng ml⁻¹ Tm (fresh weight). In particular, for AtGFAT1 OE and RNAi lines, qRT-PCR analysis and measurement of HBP intermediates were conducted each time on all three independent transgenic lines. Values represent means ±SE of three independent repeats.