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. 2019 Feb 18;70(6):1775–1787. doi: 10.1093/jxb/erz055

Fig. 7.

Fig. 7.

Symptoms of Arabidopsis plants due to GlcN deficiency. (A) Photographs of representative plants with the indicated genotype grown on GlcN-sufficient or -free medium. Seedlings were grown for 5 d on GlcN-sufficient medium (500 µM GlcN), followed by transfer to either GlcN-sufficient medium or GlcN-free medium, and then photographed 3, 5, and 10 d later. Five-day-old seedlings grown on GlcN-sufficient medium were transferred to either GlcN-sufficient medium or GlcN-free medium for 5 d and then stained with DAB (B) and trypan blue (C). (D) Reduction of protein N-glycosylation due to AtGFAT1 deficiency. Five-day-old seedlings grown on GlcN-sufficient medium were transferred to GlcN-free medium for 3 d. Seedlings grown for the same period only on GlcN-sufficient medium were used as a control. Total proteins from of these seedlings were subjected to Con A blot analysis. The pattern of Coomassie Blue staining was used as a loading control. Compared with those in qrt1-4/qrt1-4 plants, bands that displayed more weakly in qrt1-4/qrt1-4 gfa1-2/gfa1-2 plants are indicated by arrows.