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. 2019 Mar 19;10:495. doi: 10.3389/fimmu.2019.00495

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Copyright © 2019 Schmitz, Tavares, Pignataro, Machado, Pacheco, dos Santos, da Silva and Sarno.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ENL skin lesions present neutrophils expressing CD64. The protein expression of CD64 on neutrophil (MPO⁺ cells) was evaluated by immunofluorescence. Skin lesion was stained for CD64 (red), myeloperoxidase (MPO; green), and nuclei (DAPI; blue). Images are representative of three independent samples of ENL patients. Co-localized areas of MPO⁺CD64⁺ cells were identified with arrows. The regulation of neutrophil CD64 expression has the potential to be useful in the ENL treatment, as well as to prevent ENL reactional episodes. Scale bars: 10 μm. Images from Leprosy Laboratory collection.