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. 2019 Mar 20;10:160. doi: 10.3389/fendo.2019.00160

Figure 6.

Figure 6

Elevated cytotoxic CD8+ T cells in 2nd trimester high Uterine artery PI and SGA decidua. Multicolored flow cytometry analysis of leukocyte subsets isolated from 2nd trimester normal or high uterine artery PI (>1.6) of decidua. Numbers of CD45+ leukocytes, CD56++ uNK, and CD68+ macrophage were no different between groups (A). CD3+ T cells gated within the CD45+ population were doubled in the high uterine artery PI decidua (B). Visiopharm Newcast image analysis system and random meander sampling was used to quantify numbers of CD4+ T cells and CD8+ T cells in 5% of the total tissue area of each decidual section. Percentages of the total cell number were calculated and are presented as box whisker plots in (C). Representative serial sections from a 15-week normal sample (upper panel) and a 17-week high uterine artery PI SGA) stained with CK7, SMA CD4 and CD8 antibodies are shown in (D) (arrows identify CD8+ T cells). *p < 0.05, **p < 0.01, n = 6 in each group. Scale bars = 20 μM, 200x magnification.