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Evolved isolates exhibit variable survival of UV irradiation. Five isolates from each population were grown in LB medium overnight and then to early exponential phase as described in Materials and Methods. Cultures were serial diluted in 1× PBS, and then 10 μl of each dilution was spot plated onto LB agar. Once spots dried, the plates were exposed to UV irradiation and then incubated overnight before imaging. The results shown are representative of two independent experiments performed.
