FIG 7.

Evolved isolates exhibit variable survival of various DNA-damaging agents. Five isolates from each population were grown in LB medium overnight and then to early exponential phase as described in Materials and Methods. Cultures were serial diluted in 1× PBS, and then 10 μl of each dilution was spot plated onto LB agar with the indicated DNA-damaging agent. Once the spots dried, the plates were incubated overnight before imaging. The results shown are representative of two independent experiments performed.