TABLE 2.

Mutations that are likely candidates for enhancing IR resistance^a

		Mutation(s) in:
Pathway	Protein	IR9	IR10	IR11	IR12
Anaerobic respiration	ArcB	N405D	D166Y, S280R, L90 fs Y71C	R100H, S74W	S24P, K547 fs
Copper metabolism	CopA	V270F		T525A	A812V
DNA metabolism	DinI	R28H, P14A	W71*	I66N	S26Y
	Nth		C203Y, E160K		C203Y, K85N
	RecA	Y294C	A290S	A290S, E286G	E19K
	RecD	A90E, L188*, L223*, P99*	N124D, Q463*, L301M	A550E, A39G, C71 fs, G307W, W534R	S92I, A271E, C103*, T568A
	RecN	K429Q, E271G, R118L	F144L	R102P, S382R	A361T, R285C, R368H, S310L
RNA polymerase	RpoB	S72N, S391P	F15L, K1200E	P535L, S574F	T600I

^aProteins listed are encoded by genes that have (i) mutations that were nonsynonymous, (ii) mutations that occurred in a gene that was mutated in at least two additional populations, (iii) mutations that achieved at least 10% abundance at some point during selection in each population in which they appeared, and (iv) mutations that remained in the population at round 50 of selection. The mutations in RecA and Nth were included since these are the only proteins with the same variant present in two populations. The boldface variants are fixed in the indicated population, the variants listed in regular typeface are present at above a 2% frequency at round 50 of selection, and underlined variants were present in the indicated lineage but have been driven extinct by round 50 of selection.