TABLE 4.
Strains used in this studya
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| MG1655 | YbhJ L54I MntP G25D RIP321 A4296380*ACG GlpR C3560455*CG GatC ACC2173360*A | 91 |
| EAW7704 (founder Δe14) | MG1655 Δe14 RbsR L92R CytR Q110stop fabI/ycjD int (G1351174*A) yifN/ppiC int (T3959934*C) | 45 |
| IR9-50 | MG1655 exposed to 50 iterative rounds of IR; mixed population | This study |
| IR10-50 | MG1655 exposed to 50 iterative rounds of IR; mixed population | This study |
| IR11-50 | MG1655 exposed to 50 iterative rounds of IR; mixed population | This study |
| IR12-50 | MG1655 exposed to 50 iterative rounds of IR; mixed population | This study |
| IR9-50-1 | Isolate from IR9-50 | This study |
| IR9-50-2 | Isolate from IR9-50 | This study |
| IR9-50-3 | Isolate from IR9-50 | This study |
| IR9-50-4 | Isolate from IR9-50 | This study |
| IR9-50-5 | Isolate from IR9-50 | This study |
| IR10-50-1 | Isolate from IR10-50 | This study |
| IR10-50-2 | Isolate from IR10-50 | This study |
| IR10-50-3 | Isolate from IR10-50 | This study |
| IR10-50-4 | Isolate from IR10-50 | This study |
| IR10-50-5 | Isolate from IR10-50 | This study |
| IR11-50-1 | Isolate from IR11-50 | This study |
| IR11-50-2 | Isolate from IR11-50 | This study |
| IR11-50-3 | Isolate from IR11-50 | This study |
| IR11-50-4 | Isolate from IR11-50 | This study |
| IR11-50-5 | Isolate from IR11-50 | This study |
| IR12-50-1 | Isolate from IR12-50 | This study |
| IR12-50-2 | Isolate from IR12-50 | This study |
| IR12-50-3 | Isolate from IR12-50 | This study |
| IR12-50-4 | Isolate from IR12-50 | This study |
| IR12-50-5 | Isolate from IR12-50 | This study |
| CB2000 | Isolate from IR-resistant evolved population IR-2-20 | 45 |
| CB3000 | Isolate from IR-resistant evolved population IR-3-20 | 45 |
| EAW792 | Founder Δe14 ArcB N405D | This study |
| EAW971 | Founder Δe14 CopA V270F | This study |
| EAW766 | Founder Δe14 DinI R28H | This study |
| JDT56 | Founder Δe14 RecA A290S | This study |
| EAW726 | Founder Δe14 RecD A90E | This study |
| EAW725 | Founder Δe14 RecN K429Q | This study |
| EAW756 | Founder Δe14 RpoB S72N/RpoC K1172I | This study |
| EAW748 | Founder Δe14 RecD A90E RecN K428H | This study |
| EAW904 | Founder Δe14 RecD A90E RpoB S72N/RpoC K1172I | This study |
| EAW781 | Founder Δe14 RecD A90E RecN K428H RpoB S72N/RpoC K1172I | This study |
| EAW972 | IR9-50-1 CopA V270 wt | This study |
| EAW1047 | IR9-50-1 DinI R28 wt | This study |
| EAW973 | IR9-50-1 RecD A90 wt | This study |
| EA1024 | IR9-50-1 RecN K429 wt | This study |
| EAW981 | IR9-50-1 RpoB S72/RpoC K1172 wt | This study |
| EAW1021 | IR9-50-1 RecD A90 wt RpoB S72/RpoC K1172 wt | This study |
| EAW1046 | IR9-50-1 RecD A90 wt RpoB S72/RpoC K1172 wt RecN K429 wt | This study |
| STB75 | Founder Δe14 ΔaraBAD | 47 |
a
Strains generated in this study were constructed as described in Materials and Methods. A “wt” designation indicates that the protein is variant at the indicated allele in the evolved isolate but has been swapped to the wild-type (MG1655) allele. An asterisk indicates that this nucleotide position is in reference to the NCBI GenBank U00096.3 reference sequence.