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. 2019 Mar 26;201(8):e00712-18. doi: 10.1128/JB.00712-18

FIG 1.

FIG 1

The ΔcysL mutant is defective in biofilm formation. (A) Biofilm formation ability of ΔcysL and ΔcysI mutants in the presence or absence of 100 μg/ml cysteine. B. subtilis strains were grown at 30°C for 48 h in 2×SGG medium with or without cysteine under static conditions. Top-down photographs of pellicle biofilms are shown. The well diameter is 34 mm. Extended images of each pellicle are also shown. Bars, 2 mm. (B) Growth profiles of the ΔcysL and ΔcysI mutants. B. subtilis strains were grown with vigorous shaking in 2×SGG medium or 2×SGG medium plus 100 μg/ml cysteine. CFU/ml were determined only for the wild-type strain and the ΔcysL mutant.