FIG 3.

Sulfite inhibits biofilm formation by killing cells. (A) Impacts of sulfite on biofilm formation. The wild-type strain was statically grown at 30°C for 18 h in 2×SGG medium, and then sulfite was added to the cultures. The cultures were further incubated at 30°C for 30 h. (B) Impact of sulfite on growth. The wild-type strain was grown to an OD₆₀₀ of 1.0, and then the culture was divided into three parts. Each of the cultures was treated with 0, 1, or 3 mM sulfite. An arrow indicates the time point at which sulfite was added to the cultures.