TABLE 1.
B. subtilis strains used in this study
| Strain | Relevant feature(s) | Reference or constructiona |
|---|---|---|
| NCIB3610 | Prototroph | 5 |
| NTF234 | ΔcysL::cat | WTF237 (17)→ NCIB3610 |
| N502 | ΔcysI::pMutin (erm) | YVGRd (48)→NCIB3610 |
| N503 | ΔcysH::pMutin (erm) | CYSHd (48)→NCIB3610 |
| N504 | ΔcysP::pMutin (erm) | CYSPd (48)→NCIB3610 |
| N505 | Δsat::pMutin (erm) | SATd (48)→NCIB3610 |
| N506 | ΔcysC::pMutin (erm) | CYSCd (48)→NCIB3610 |
| N507 | ΔylnD::pMutin (erm) | YLNDd (48)→NCIB3610 |
| N508 | ΔylnE::pMutin (erm) | YLNEd (48)→NCIB3610 |
| N509 | ΔylnF::pMutin (erm) | YLNFd (48)→NCIB3610 |
| N510 | ΔcysH::pMutin (erm) ΔcysL::cat | N503→NTF234 |
| N511 | ΔcysP::pMutin (erm) ΔcysL::cat | N504→NTF234 |
| N512 | Δsat::pMutin (erm) ΔcysL::cat | N505→NTF234 |
| N513 | ΔcysC::pMutin (erm) ΔcysL::cat | N506→NTF234 |
| N514 | ΔylnD::pMutin (erm) ΔcysL::cat | N507→NTF234 |
| N515 | ΔylnE::pMutin (erm) ΔcysL::cat | N508→NTF234 |
| N515 | ΔylnF::pMutin (erm) ΔcysL::cat | N509→NTF234 |
| N519 | trxA:pMutinNC (Pspac-trxA erm) | TRXAp (48)→NCIB361 |
| N520 | trxA:pMutinNC (Pspac-trxA erm) ΔcysL::cat | N519→NTF234 |
| N523 | Δspx::kan | This study |
| N524 | Δspx::kan ΔcysL::cat | N520→NTF234 |
| N744 | ΔyjbH::pMutinNC (erm) | This study |
| N751 | ΔyjbH::pMutinNC (erm) Δspx::kan | N744→N523 |
| N743 | ΔyjbH::kan | This study |
| N440 | ΔsrfA operon | This study |
| NTF2 | ΔabrB::kan | WTF2 (17)→NCIB3610 |
| N552 | ΔabrB::kan ΔcysL::cat | NTF2→NTF234 |
| NTF92 | ΔsinR::cat | WTF92 (17)→NCIB3610 |
| N553 | ΔsinR::kan ΔcysL::cat | NTF92→NTF234 |
| N901 | epsA::pMutinT3-hy (Pspac-hy-epsABCDEFGHIJKLMNO erm) | This study |
| N902 | epsA::pMutinT3-hy (Pspac-hy-epsABCDEFGHIJKLMNO erm) ΔcysL::cat | NTF234→N901 |
| N903 | epsA::pMutinT3-hy (Pspac-hy-epsABCDEFGHIJKLMNO erm) ΔyjbH::kan | N743→N901 |
| N963 | tapA::pCAxylAtapA (PxylA-tapA-sipW-tasA spc) | This study |
| N964 | tapA::pCAxylAtapA (PxylA-tapA-sipW-tasA spc) ΔcysL::cat | NTF234→N963 |
| N904 | bslA::pMutinT3-hy (Pspac-hy-bslA tet) | This study |
| N905 | bslA::pMutinT3-hy (Pspac-hy-bslA tet) ΔcysL::cat | NTF234→N904 |
| N978 | ΔepsH::pMutin (epsA-H-lacZ erm) | YVERd (48) →NCIB3610 |
| N979 | ΔepsH::pMutin (epsA-H-lacZ erm) ΔcysL::cat | NTF234→N978 |
| N980 | ΔepsH::pMutin (epsA-H-lacZ erm) ΔyjbH::kan | N743→N978 |
a
Arrows indicate B. subtilis transformation, donor strain → recipient strain.