FIG 2.
Ability of GFP-DedD and variants to rescue DedD-depleted ftsNslm117 cells. Strain MG19/pMG39 [ΔdedD ftsNslm117/PBAD::dedD] carrying one of the indicated plasmids was grown overnight in LB with 0.5% arabinose. Cultures were serially diluted in LB to an OD600 of 4 × 10x, and 5 μl of each dilution was spotted on LB agar containing arabinose, IPTG, or neither, as indicated. The plates were incubated for 16 h. Save for the vector control, pMLB1113ΔH [Plac::], each plasmid encoded GFP-DedD or a mutant derivative under control of the lac regulatory region. The relevant plasmid name and encoded protein are indicated on the left and right of each row, respectively. Plasmids pFB236 and pBL272 are almost identical, except that the GFP-DedD fusion encoded by the latter carries an additional four residues in the linker peptide that connects the GFP and DedD moieties.