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Interactions of DedD1–118 and mutant variants with other division proteins in BACTH assays. Strain BTH101 [cya] was cotransformed with the plasmid pairs indicated at the right and bottom and plated on LB agar containing Amp, Kan, and 0.2% glucose. Purified colonies were subsequently striped on M9-glucose agar supplemented with Amp, Kan, 40 μg/ml 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal), and 250 μM IPTG. The plate was incubated for 48 h before imaging.
