Skip to main content
. 2019 Mar 26;201(8):e00577-18. doi: 10.1128/JB.00577-18

FIG 3.

FIG 3

BSAP-4 receptor analysis. (A) ORF maps of the four distinct genetic variants in the bsap-4 or corresponding genetic regions of sequenced B. fragilis strains. Genes highlighted in purple are conserved between genomes. Red indicates the bsap-4 MACPF gene. Yellow and green genes identify the three OMP variants. Lines between genomes delineate the extent of the major divergences. (B) Each of the three OMP variant proteins shown as a line with the extent of the calycin-like domains (PF13944.5) shown above in pink with the amino acid positions of the domains indicated. (C) Agar overlay assays showing that the deletion of the variant 2 OMP gene in strain J38-1 (J38-1 Δ2717) renders the strain resistant to BSAP-4 and that sensitivity to BSAP-4 is restored when the OMP gene is added to the mutant in trans (p2717).