FIG 4.

Analysis of the growth inhibition of strains when distinct variant 1, 2, and 3 OMP genes are constitutively expressed in trans. (A) A variant 1 OMP (638R), a variant 3 OMP (3_1_12), and each of the five distinct variant 2 OMPs were cloned into a vector for constitutive expression and placed in trans in B. thetaiotaomicron. Compared to vector alone, the variant 3 and all variant 2 OMPs conferred BSAP-4 sensitivity to B. thetaiotaomicron, but the variant 1 OMP did not. (B) The same analysis as in panel A except that the OMP-encoding plasmids were transferred to 638R Δbsap-1 Δubb Δbsap-4 (638RΔΔΔ). (C) Constitutive expression of the CL03T00C08 OMP, which is identical to that of the background strain I1345, confers a degree of BSAP-4 sensitivity to this otherwise resistant strain.