TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| S. Typhimurium | ||
| SL1344 | Wild type; xyl hisG rpsL Smr | 74 |
| JPTM3 | ΔompR::kan | 18 |
| JPTM5 | ΔhilD::kan | 18 |
| JPTM8 | ssrA::3×FLAG-kan | 18 |
| JPTM25 | ΔhilD | 75 |
| JPTM28 | ΔompR | 75 |
| DTM99 | ΔssrB | 62 |
| DTM104 | ΔphoP | 44 |
| DTM115 | ΔslyA::kan | This study |
| DTM116 | ΔslyA | This study |
| DTM117 | ΔhilD ΔslyA::kan | This study |
| DTM118 | ΔhilD ΔslyA | This study |
| DTM119 | ΔslyA ssrA::3×FLAG-kan | This study |
| DTM120 | ΔslyA ssrA::3×FLAG | This study |
| E. coli | ||
| M15 | Strain for expression of recombinant proteins | Qiagen |
| DH10β | Laboratory strain | Invitrogen |
| Plasmids | ||
| pKK232-8 | pBR322 derivative containing a promoterless chloramphenicol acetyltransferase (cat) gene, Apr | 76 |
| pssrAB-cat-302 + 478 | pKK232-8 derivative containing a ssrAB-cat transcriptional fusion from nucleotides −302 to +478 | 19 |
| pssrAB-cat-302 + 10 | pKK232-8 derivative containing a ssrAB-cat transcriptional fusion from nucleotides −302 to +10 | 19 |
| pssaG-cat | pKK232-8 derivative containing a ssaG-cat transcriptional fusion from nucleotides −303 to +361 | 18 |
| ppagK-cat | pKK232-8 derivative containing a pagK-cat transcriptional fusion from nucleotides −880 to +251 | 44 |
| pMPM-K3 | p15A derivative low-copy-number cloning vector, lac promoter, Kanr | 71 |
| pK3-SlyA | pMPM-K3 derivative expressing SlyA from the lac promoter | This study |
| pK3-SsrB | pMPM-K3 derivative expressing SsrB from the lac promoter | 62 |
| pK3-PhoP | pMPM-K3 derivative expressing PhoP from the lac promoter | 44 |
| pMPM- T6Ω | p15A derivative low-copy-number cloning vector, arabinose-inducible promoter, Tcr | 71 |
| pT6-HNS-WT | pMPM-T6Ω derivative expressing promoter WT H-NS from the arabinose-inducible | 18 |
| pT6-HNS-G113D | pMPM-T6Ω derivative expressing H-NSG113D from the arabinose-inducible promoter | 44 |
| pQE30 | Vector for expression of recombinant proteins, lac promoter, Apr | Qiagen |
| pQE30-His-HA-SlyA | pQE30 derivative expressing His-HA-SlyA from the lac promoter, Apr | This study |
| pBAD-H-NS-FH | pBADMycHisC derivative expressing H-NS−FH from an ara promoter, Apr | 44 |
| pKD46 | pINT-ts derivative expressing red recombinase under the control of an arabinose-inducible promoter, Apr | 72 |
| pKD4 | pANTsγ derivative template plasmid containing the kanamycin cassette for λRed recombination, Apr | 72 |
| pCP20 | Plasmid expressing FLP recombinase from a temperature-inducible promoter, Apr | 72 |
a
The coordinates for the cat fusions are indicated with respect to the transcriptional start site of ssrA, ssaG, or pagK. Smr, streptomycin resistance; Apr, ampicillin resistance; Kanr, kanamycin resistance; Tcr, tetracycline resistance.