TABLE 2.
Primers used in this work
| Primer by use | Sequence (5′–3′)a | Target gene | REb |
|---|---|---|---|
| Gene cloning | |||
| SlyA-RV11 | ACGGGATCCTCGGCAGGTCAGCGTGTCG | slyA | BamHI |
| SlyA-FW22 | TAAAAGCTTAGCAAGCTAATTATAAGGAG | slyA | HindIII |
| SlyA-HA-His-F | GATGGATCCTCTATCCGTATGATGTTCCTG ATTATGCTAGCCAAATTCGAATCGCCACTA GGTTC | slyA | BamHI |
| SlyA-HA-His-R | CTAAAGCTTTGTCGTGCTCGCCAGCAACG | slyA | HindIII |
| EMSAs | |||
| SsaBF (fw) | GGCTAAGATCTTCGGCCCTGATATCCTG | ssrAB | |
| SsrBRS6E (rv) | TTGGTCGACCGACAGATAGATGCCGG | ssrAB | |
| Gene deletions | |||
| slyA-H1P1 | GCTAATTATAAGGAGATGAAATTGGAATC GCCACTAGGTTGTAGGCTGGAGCTGCTT CG | slyA | |
| slyA-H2P2 | GTATGCCCCTGCACCTCAATCGTGAGAG TGCAATTCCATCATATGAATATCCTCCTT AG | slyA |
a
Underlined letters indicate the respective restriction enzyme site in the primer. The sequences corresponding to the template plasmid pKD4 (Table 1) are in italics.
b
RE, restriction enzyme for which a site was generated in the primer.