Table 2.
Path of steepest ascent experimental design and results.
| Run | Factor 1 | Factor 2 | Response |
|---|---|---|---|
| Number | Ethanol (%) |
Flavonoid (mg/50mL) |
|
| null point(0) | 1 | 70 | |
| step length(Δ) | 1 | 1.5 | |
| 1 | 1 | 70 | 12.94 |
| 2 | 2 | 71.5 | 20.26 |
| 3 | 3 | 73 | 22.67 |
| 4 | 4 | 74.5 | 28.49 |
| 5 | 5 | 76 | 30.27 |
| 6 | 6 | 77.5 | 33.81 |
| 7 | 7 | 79 | 31.13 |