Figure 2.

Representative proton MR spectroscopy at the left proximal femur of a rabbit from the SHAM control at baseline conditions, 2.5 and 5 months [(A), FF = 42.9%; (B), FF = 42.5%; (C), FF = 42.7%], OVX control at baseline condition, 2.5 and 5 months [(D), FF = 43.1%; (E), FF = 50.1%; (F), FF = 56.5%, respectively), and Scl-Ab-treated group at baseline condition, 2.5 and 5 months [(G), FF = 43.5%; (H), FF = 43.6%; (I), FF = 44.0%, respectively]. OVX, ovariectomy; Scl-Ab, sclerostin antibody; Sham, sham-operation. Bulk CH₂ methylene protons (labeled L13 at ~1.3 ppm), the CH₂ methylene protons α- (L23 at ~2.3 ppm), water (W at ~4.7 ppm), and olefinic protons overlapped with the glycerol CH methine proton (L52 at ~5.20 ppm). CH₃ methyl protons (0.9 ppm) and bulk CH₂ methylene protons (1.3 ppm) appeared as a single peak at clinical field strengths (≤3T MRI).