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. 2019 Mar 20;10:260. doi: 10.3389/fpls.2019.00260

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Copyright © 2019 Top, Parsons, Bohlender, Michelfelder, Kopp, Busch-Steenberg, Hoernstein, Zipfel, Häffner, Reski and Decker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification of mossMFHR1. (A) Coomassie staining of MFHR1 elution fractions 8-13, purified via Ni-NTA chromatography. (B) Western blot of the same elution fractions detected with anti-His-tag antibodies. MW1: PageRuler Prestained Protein Ladder (Thermo Fisher Scientific). (C) Coomassie staining of MFHR1 elution fraction pools (E8-9 and E10-16) and BSA as a reference. MW2: Precision Plus Protein™ Standard (BioRad).