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. 2019 Mar 20;10:260. doi: 10.3389/fpls.2019.00260

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Copyright © 2019 Top, Parsons, Bohlender, Michelfelder, Kopp, Busch-Steenberg, Hoernstein, Zipfel, Häffner, Reski and Decker.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MossMFHR1 displays decay acceleration activity on the C3 convertase C3bBb. Like mossFH and plasmaFH, mossMFHR1 accelerated the decay of Bb from C3 convertase C3bBb complex in a dose-dependent manner. The OD at 450 nm (OD₄₅₀) of preformed C3bBb (C3b + FB + FD) without any regulator was set to 100%. C3bBB OD₄₅₀ (only C3b + FB without any FD and regulator) was set to 0%. Purified extract of the parental moss strain Δxt/ft was included as a control, in equal amounts to the volume of MFHR1 used for the highest concentration. Data represent mean values and ± SD from three replicates and were analyzed with two-way ANOVA followed by Bonferroni test. Some error bars are shorter than the symbol. Analyses were done with GraphPad Prism software version 8.0 for Windows.