Table 3.

Predicted NAD-Binding Affinity of the Eight Putative NAD Binders

protein name	gene ID	PDB ID	NAD-binding affinity (kcal/mol)	binding affinity of the cognate ligand (kcal/mol)
galactose-1-phosphate uridylyltransferase	galT	1GUQ	−11.0	−12.5
5′-methylthioadenosine and S-adenosylhomocysteine nucleosidase	mtn	3O4V	−10.6	−10.4
purine nucleoside phosphorylase	deoD	1K9S	−10.5	−9.4
disulfide reductase and organic hydroperoxide reductase	yghU	3C8E	−10.2	−6.2
peptidyl–prolyl cis–trans isomerase A	ppiA	1V9T	−8.2	−7.7
3-methyl-2-oxobutanoate hydroxymethyltransferase	panB	1M3U	−8.2	−6.5
malonyl–coA–ACP transacylase	fabD	1MLA	−7.3	−6.0
UDP–sugar hydrolase	ushA	1HP1	−7.2	−6.6

^aThe affinity was computed with Autodock Vina.