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. Author manuscript; available in PMC: 2019 Sep 26.
Published in final edited form as: J Am Chem Soc. 2018 Sep 18;140(38):11974–11981. doi: 10.1021/jacs.8b05012

Figure 4.

Figure 4.

Targeted YTHDF2 readers to study the regulation of endogenous methylated transcripts in live cells. (A) HEK293T cells were transfected with dCas13b-YTHDF2 and either NT gRNA (gray bar) or KRAS gRNA (red bar). After 48 h, mRNA levels were determined by RT-qPCR (B) The same analysis was performed in (A) except the nuclease active Cas13b was delivered. (C) The same analysis was performed in (A) except a dCas13b protein without a fusion was delivered. (D) HEK293T cells were transfected with dCas13bYTHDF2 and either NT gRNA (gray bar) or PPIB gRNA (red bar). After 48 h, mRNA levels were determined by RT-qPCR (E) The same analysis was performed in (D) except the nuclease active Cas13b was delivered. (F) The same analysis was performed in (D) except a dCas13b protein without a fusion was delivered. Student’s t-test; *P < 0.05, ***P < 0.001 (n = 3).